Coding

Part:BBa_K4762001:Design

Designed by: Xuan Hang   Group: iGEM23_NWU-CHINA-A   (2023-10-11)


CAT（Catalase）


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1218
    Illegal PstI site found at 1051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1218
    Illegal NheI site found at 1033
    Illegal PstI site found at 1051
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1218
    Illegal BamHI site found at 15
    Illegal BamHI site found at 1468
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1218
    Illegal PstI site found at 1051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1218
    Illegal PstI site found at 1051
    Illegal AgeI site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our CAT sequence information comes from NCBI and is codon optimized for its role in E. coli


Source

The sequence information of CAT comes from human chromosome 11, and the CAT gene used in this team was synthesized by Genscript Biotech Corporation

References